Protective effect of curcumin on lipopolysaccharide-induced rat cardiomyocyte hypertrophy
By: Lu, Yuanzhou
.
Contributor(s): Gong, Wei.
Publisher: Mumbai Indian Journal of Pharmaceutical Science 2022Edition: Vol.84(5), Sep-Oct.Description: 1133-1139p.Subject(s): PHARMACEUTICSOnline resources: Clikc here
In:
Indian journal of pharmaceutical sciencesSummary: To study the protective effect of curcumin on lipopolysaccharide-induced rat cardiomyocyte hypertrophy is
the main objective of the study. Cardiomyocytes were purified and cultured by differential adherence in in
vitro and then they were divided into control group, lipopolysaccharide group, lipopolysaccharide+curcumin
low-dose (12.5 mg/l) group, lipopolysaccharide+curcumin medium-dose (25 mg/l) group and
lipopolysaccharide+curcumin high-dose (50 mg/l) group. The morphological changes were observed under
a phase-contrast inverted microscope and the cell volume was measured. The total protein content of the
cell was detected by Coomassie brilliant blue kit. Enzyme-linked immunosorbent assay method was used to
detect the level of tumor necrosis factor alpha. The TILL imaging system was used to observe the changes
in intracellular calcium ions. Compared with the control group, lipopolysaccharide significantly increased
the cardiomyocyte volume, the total cell protein content, the level of tumor necrosis factor alpha (p<0.01)
and the instantaneous peak of calcium ions. Compared with the lipopolysaccharide group, 12.5, 25, 50 mg/l
curcumin treatment inhibited the increase of cardiomyocyte volume, total protein content, tumor necrosis
factor alpha level and the instantaneous peak of calcium ions induced by lipopolysaccharide and showed
a dose-dependent effect. Curcumin has a protective effect on rat cardiomyocyte hypertrophy induced by
lipopolysaccharides, which may be related to the inhibition of tumor necrosis factor alpha production and
the inhibition of calcium ions overload.
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
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Articles Abstract Database
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School of Pharmacy Archieval Section | Not for loan | 2023-1122 |
To study the protective effect of curcumin on lipopolysaccharide-induced rat cardiomyocyte hypertrophy is
the main objective of the study. Cardiomyocytes were purified and cultured by differential adherence in in
vitro and then they were divided into control group, lipopolysaccharide group, lipopolysaccharide+curcumin
low-dose (12.5 mg/l) group, lipopolysaccharide+curcumin medium-dose (25 mg/l) group and
lipopolysaccharide+curcumin high-dose (50 mg/l) group. The morphological changes were observed under
a phase-contrast inverted microscope and the cell volume was measured. The total protein content of the
cell was detected by Coomassie brilliant blue kit. Enzyme-linked immunosorbent assay method was used to
detect the level of tumor necrosis factor alpha. The TILL imaging system was used to observe the changes
in intracellular calcium ions. Compared with the control group, lipopolysaccharide significantly increased
the cardiomyocyte volume, the total cell protein content, the level of tumor necrosis factor alpha (p<0.01)
and the instantaneous peak of calcium ions. Compared with the lipopolysaccharide group, 12.5, 25, 50 mg/l
curcumin treatment inhibited the increase of cardiomyocyte volume, total protein content, tumor necrosis
factor alpha level and the instantaneous peak of calcium ions induced by lipopolysaccharide and showed
a dose-dependent effect. Curcumin has a protective effect on rat cardiomyocyte hypertrophy induced by
lipopolysaccharides, which may be related to the inhibition of tumor necrosis factor alpha production and
the inhibition of calcium ions overload.
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